![]() ** For PrecisA Monoclonals use OmniMap anti-Ms HRP. * For Triple A Polyclonals use OmniMap anti-Rt HRP. Coverslip the slides in permanent mounting media. Wash the slides in warm tap water with detergent and dehydrate in graded ethanol and xylene. Post counterstain with Bluing Reagent, incubate for 8 minutes.Counterstain with Hematoxylin, incubate for 8 minutes. ![]() Apply one drop of Copper CM, incubate 5 minutes.Apply one drop of DAB CM and One Drop H 2O 2 CM, incubate 8 minutes.Apply one drop of OmniMap anti-Ms HRP and incubate for 16 minutes**.Apply one drop of OmniMap anti-Rb HRP and incubate for 16 minutes*.Incubation with primary antibody for 60 minutes.Block with Inhibitor CM, 37☌ 4 minutes.Cell Conditioning using Conditioner #1, Standard CC1, 95☌ 44 minutes.Deparaffinization in EZ prep 75☌ 8 minutes.Washing buffer between the steps is Reaction buffer.Īpply 100 µl volume of primary and secondary antibodies. IHC staining protocol Ventana Discovery XT Optimal dilutions must be determined by end-user. NOTE: The specified working dilutions of the primary antibodies are to be considered as a guideline only. Blocking stepsįor blocking endogenous peroxides and protein, these steps are done in the Ventana Discovery XT platform using Inhibitor CM. If expected results are not achieved, test a different time or use Citrate-based buffer pH 6.0 or Protease antigen retrieval instead. The standard antigen retrieval method is Heat Induced Epitope Retrieval (HIER) in Tris-EDTA buffer pH 7.8 at 95☌ for 44 min (standard CC1) and is performed in the Ventana Discovery XT. The deparaffinization step is done in the Ventana Discovery XT platform using EZ prep solution. Paraffin sections of 4 µm thickness are baked overnight at 50☌. “Quick results are crucial in the fight against cancer, and every additional day that a clinician and a patient must wait for test results is a day too long.Here you can download a pdf version of the IHC Ventana Discovery XT protocol. Roche Diagnostics CEO Michael Heuer said: “The new VENTANA HER2 Dual ISH assay advances Roche’s commitment to personalized healthcare by delivering critical information on treatment options for breast and gastric cancer patients faster. Roche offers a comprehensive menu of both diagnostic and predictive assays, including the Ventana HER2/neu (4B5) rabbit monoclonal primary antibody that will help assess breast cancer patients for whom Herceptin treatment is considered. Herceptin binds to a specific section of the HER2 protein, restricting the signals it sends that advance tumor cell growth and also alerts body’s immune system to attack the cancer cells. Herceptin is a humanized monoclonal antibody, which will target and inhibit the function of the HER2 receptor, a protein found on the outside of many normal cells and in high quantities on the outside of cancer cells in HER2-positive cancers. The company is also planning to submit an application with the US Food and Drug Administration for approval. It quickly offers clear results to pathology labs, enabling to make treatment decisions earlier.Īt present, the assay is available in Europe, the Middle East and Africa, as well as Latin America and Asia Pacific. The new Ventana HER2 Dual ISH DNA Probe Cocktail assay can be fully automated on the BenchMark IHC/ISH instruments. Roch claims that it is the only provider of a fully automated brightfield Dual ISH solution to detect HER2 amplification. Results can be read using light microscopy, helping to avoid the use of a specialized fluorescence microscope. Ventana HER2 Dual ISH DNA Probe Cocktail assay can be completed within the same day, offering clinicians with quicker results compared to the most common methods of confirmatory testing for HER2. Human epidermal growth factor receptor 2 (HER2) is a significant biomarker observed in breast and gastric cancers. Image: The administration and R&D buildings, Roche Diagnostics, Rotkreuz. Roche has introduced new Ventana HER2 Dual ISH DNA Probe Cocktail assay to detect HER2 biomarker in breast and gastric cancer patients.
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